AHF Zambia began its work in 2007 at Chikankata Mission Hospital in the Southern Province. Since then, it has partnered with government and community organizations to expand HIV testing and treatment programs across 12 districts.

AHF Zambia operates mobile testing units offering free rapid HIV testing and condom distribution at public places like markets and bus stations. Through partnerships with local organizations like DAPP, AHF Zambia offers door-to-door testing and counseling, with a focus on index testing, and links those who test positive to care. Additional services include laboratory testing, pharmacies, and training future healthcare workers to care for people living with HIV/AIDS.

In 2009, AHF Zambia introduced a mobile testing unit to deliver HIV testing, treatment, and condoms to hard-to-reach communities. In 2012, the organization launched the Provider Initiated Counseling and Testing (PICT) program to increase HIV testing by training volunteer counselors who conduct testing at inpatient health clinics. As of December 2024, AHF Zambia provides care and treatment to nearly 158,000 clients. The program is focused on expanding its geographic reach, training more healthcare workers, and further developing the infrastructure of its existing sites.