In September 2016, AHF and the Zimbabwe Ministry of Health and Child Care signed a memorandum of understanding to implement programs supporting the national health strategy for HIV and AIDS prevention, treatment, and community services. The initial phase established Centers of Excellence at Parirenyatwa and Mpilo Referral Hospitals and has since expanded to include facilities like Sally Mugabe Central Hospital, Gweru Provincial Hospital, Victoria Chitepo Provincial Hospital, Masvingo General Hospital, and Gwanda Provincial Hospital.  

AHF also supports primary care facilities in Harare, Mutare, Gweru, and Bulawayo, as well as the Chikurubi Maximum Prison OI Clinic. To address the spread of STIs in the area, the program expanded in 2024 to two more provincial hospitals, a prison site, and a wellness center in Glen View, Harare. Additionally, AHF collaborates with MoHCC to prevent new HIV infections by increasing awareness of HIV status and treatment, offering both in-facility and outreach testing, and ensuring immediate linkage to care for positive clients.

AHF supports sites in six major towns across Zimbabwe, covering six of the country's 10 provinces. Over the past few years, AHF has conducted targeted testing, with a positivity rate consistently above 6%. The Country Office also plays a key role in AHF's marketing efforts, participating annually in major national events, such as the Zimbabwe Medical Association conference, ZITF, the Zimbabwe Agricultural Show, and provincial exhibition shows, where it provides free HIV testing services. Since August 2017 through December 2023, AHF distributed more than 10.8 million free condoms.  

AHF provides comprehensive services, including antiretroviral therapy, viral load testing, and counseling on a wide range of sexual and reproductive health issues. It offers adolescent-friendly services, support groups for people living with HIV, viremia clinics, and HIV testing services. Additionally, AHF supports selected clients with medical costs outside of care at supported sites, including transport costs, and provides basic necessities like food to vulnerable clients.  

Recently, AHF revamped its Food for Health projects by encouraging sites with available space to partner with clients in market gardening, allowing them to grow crops for resale.