AHF Chile was founded in October 2018 in Santiago City after visits and conversations with local NGOs by Dr. Patricia Campos, AHF Chief of the Latin American & the Caribbean Bureau. The first facility was an HIV testing site located near the historical neighborhood of Santiago.

AHF Chile offers prevention, testing, and linkage to care services, helping undocumented migrants living with HIV access treatment through the National Health System by supporting their enrollment in health insurance. AHF Chile is present in the Metropolitan Region and has expanded its reach by opening new testing sites in the 8th Region (Concepción) and the 2nd Region (Antofagasta), in addition to a Wellness Center. Across these locations, the team conducts approximately 10,000 HIV tests annually.

A prominent NGO within the LGBTQIA+ community, AHF Chile also provides a safe and welcoming space for HIV services. It has led the rollout of HIV rapid testing in the country and has actively contributed to a working group for law reform, partnering with other NGOs to strengthen national HIV public policies.